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Image Search Results
Journal: International Journal of Clinical and Experimental Medicine
Article Title: Chinese medicine Jinlida (JLD) ameliorates high-fat-diet induced insulin resistance in rats by reducing lipid accumulation in skeletal muscle
doi:
Figure Lengend Snippet: Primer sequences for quantitative polymerase chain reaction
Article Snippet: Membranes were incubated with the appropriate diluted
Techniques: Amplification
Journal: International Journal of Clinical and Experimental Medicine
Article Title: Chinese medicine Jinlida (JLD) ameliorates high-fat-diet induced insulin resistance in rats by reducing lipid accumulation in skeletal muscle
doi:
Figure Lengend Snippet: The effects of Jinlida on AMPK signaling and adiponectin receptor gene expression in skeletal muscle in rats. The mRNA expression levels of adiponectin receptor 1 (ADIPOR1), adiponectin receptor 2 (ADIPOR2), AMP-activated protein kinase (AMPK), Acetyl-CoA carboxylase (ACC) and glucose transporter type 4 (GLUT4) were examined by quantitative real-time-polymerase chain reaction (A-F). The corresponding bands of western blotting were showed (H).The protein levels of P-AMPK, P-ACC, AMPK, ACC and GLUT4 were detected by Western blotting (G-K). We calculated the expression of proteins as [(OD target protein/OD β-actin) experiment group]/[(OD target protein/OD β-actin) control group]. So the standard deviation in ND group was 1. n = 6 per group; *P < 0.05 compared with the HFD group; #P < 0.05 compared with the ND group.
Article Snippet: Membranes were incubated with the appropriate diluted
Techniques: Gene Expression, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Standard Deviation